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KMID : 1237720110440020106
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Anatomy & Cell Biology
2011 Volume.44 No. 2 p.106 ~ p.115
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Immunohistochemical study on the expression of calcium binding proteins (calbindin-D28k, calretinin, and parvalbumin) in the cerebral cortex and in the hippocampal region of nNOS knock-out(-/-) mice
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Cho Yu-Jin
Lee Jae-Chul
Kang Bong-Gu
An Jae-Yeol
Song Hyeon-Suk
Son On-Ju
Nam Do-Hyun
Cha Choong-Ik
Joo Kyeung-Min
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Abstract
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Nitric oxide (NO) modulates the activities of various channels and receptors to participate in the regulation of neuronal intracellular Ca2+ levels. Ca2+ binding protein (CaBP) expression may also be altered by NO. Accordingly, we examined expression changes in calbindin-D28k, calretinin, and parvalbumin in the cerebral cortex and hippocampal region of neuronal NO synthase knockout(-/-) (nNOS-/-) mice using immunohistochemistry. For the first time, we demonstrate that the expression of CaBPs is specifically altered in the cerebral cortex and hippocampal region of nNOS-/- mice and that their expression changed according to neuronal type. As changes in CaBP expression can influence temporal and spatial intracellular Ca2+ levels, it appears that NO may be involved in various functions, such as modulating neuronal Ca2+ homeostasis, regulating synaptic transmission, and neuroprotection, by influencing the expression of CaBPs. Therefore, these results suggest another mechanism by which NO participates in the regulation of neuronal Ca2+ homeostasis. However, the exact mechanisms of this regulation and its functional significance require further investigation.
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KEYWORD
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Calcium binding proteins, Cerebral cortex and hippocampal region, Immunohistochemistry, Neuronal nitric oxide synthase (nNOS), nNOS knock-out(-/-) mice
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